عنوان مقاله [English]
نویسنده [English]چکیده [English]
Birch (Betula sp.) is considered as a survivor of Caspian primary forests and one of the extincting forest trees in Iran. In our country birch species endengred because of limited range distribution, Unfavorable habitat conditions, lake of regeneration in natural habitat and Inability to reproduce sexually. Therefore using the techniques of in vitro is very important to support this species. The objective of the present research Optimization of culture medium and surface sterilization treatments for B. litwinowii micropropagation that is one of known Birch by use of nodal explants. Nodal explants were collected randomly from 4-5 years old of birch seedlings and sterilized by applying different treatments in both MS and WPM medium with various concentrations of hormones BAP and NAA were cultured. The obtained data were analyzed based on completely randomized design using SPSS and MSTATC software. The results revealed that HgCl2 (0.1% for 7 min) was the best treatment for surface sterilization of nodal explants. The MS and WPM media supplemented with various concentrations of hormones were suitable for culture establishment of nodal explants. More than 70 % of single lateral bud nodal in both the medium becomes to leaves, finally, 10% of nodales able to produce shoot and roots in WPM medium.