Study of differentiation of active components of Fraxinus excelsior and its relationship with physical and chemical properties of soil in Hyrcanian forests

Document Type : Complete scientific research article

Authors

1 1Ph.D. Student, Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

2 Professor, Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

3 Associate Prof., Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

4 Decomposition Chemistry Group, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

Abstract

Abstract1
Background and Objectives: Comon Ash is a forest species with versatile biological and medicinal activities that extracts of its various organs are used as a model for making new compounds in the treatment of various human diseases. There is a high correlation between the geographical origin of medicinal plants and the active compounds produced in them. Therefore In this study, using this correlation, the most suitable habitat in terms of production of secondary metabolites is introduced

Materials and Methods: For this research, selected eight habitats from an altitude range of 200 to 1600 meters (Perchink, three parcels of Panj Amreh series, Alandan, Lala, Qarnsara and Ilal) in Tajan and Siahroud watersheds located in Mazandaran province. In each habitat, 3 tree that were similar to each other in terms of quality characteristics were selected (tree diameter and height, leaf freshness and health, canopy density). Leaf samples from the middle part of the branches. The canopy was harvested in four directions in order to eliminate the effect of geographical direction in sampling. The leaf samples were dried in the open air and after grinding and preparing the extract with absolute ethanol, the leaf extracts were prepared for injection by gas chromatography. In order to study the physical and chemical properties of the soil, a soil sample was taken in combination around each tree base from a depth of 0-15 cm. Then physicochemical properties of soil such as bulk density, acidity, organic crane, total nitrogen, available phosphorus, potassium and calcium were measured in the laboratory. One-way analysis of variance was used to investigate the differences between the sampling areas in terms of secondary chemical composition in the leaves. Pearson double correlation was also used to find out how environmental and soil parameters relate to these chemical compounds.

Results: The results showed that the characteristics of the sampling location had an effect on the values of the identified secondary compounds. All identified compounds (except Vitamin E and Ferruginol) had a positive correlation with altitude factor. The highest levels of Neophytadiene (27.7%), Squalene (21.7%), n-Hexadecanoic acid (26.8%), Octadecatrinoic acid (28.3%), Phytol (6.35%) and Benzeneethanol (10.39%) were observed in ilal. There was no significant relationship between soil physical properties such as moisture content, bulk density and soil texture with chemical compounds in the leaves. Also, in all studied habitats, there was a significant negative relationship between soil nutrients and identified compounds. Also, a number of identified compounds were observed in only one or more habitats and were not common in all habitats studied.

Conclusion: The results of this study showed with increasing altitude, the amount of metabolites in the leaves increased and most of the soil nutrients had a negative correlation with the identified chemical compounds. In other words, reducing soil nutrients can cause some kind of stress and increase the production of secondary metabolites in the plant.

Keywords

References

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Journal of Wood and Forest Science and Technology
Volume 27, Issue 3
December 2020
Pages 131-148

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