Producing and evaluating of Bacterial Nano- cellulose (BNC) using Acetobacter xylinum bacteria

Document Type : Complete scientific research article

Authors

1 M.Sc. Student in Pulp and Paper Industry, Gorgan University of Agricultural Sciences and Natural Resources

2 Assistant Professor, Paper Science and Engineering Department, Wood and Paper Engineering faculty, Gorgan University of Agricultural Sciences and Natural Resources

3 Associate Professor Paper Science and Engineering Department, Wood and Paper Engineering faculty, Gorgan University of Agricultural Sciences and Natural Resources

Abstract

Background and objective: Bacterial Nano-cellulose (BNC) due to special microscopic structures has a high potential in special used (such as medical and sanitary applications). BNC differs from plant cellulose in its higher purity, crystallinity, and degree of polymerization. The goal of this study was to investigate the production of BNC using Acetobacter xylinum bacteria and its characteristics evaluation.
Materials and methods: In order to obtain BNC, the bacterium was cultured in a liquid culture medium under static and dynamic conditions and the resulting cellulose was washed and purified. Microstructures with field emission scanning electron microscopy (FE-SEM), crystallinity structure by X-ray diffraction (XRD), weight percentage, size and distribution of BNC with energy-dispersive X-ray spectroscopy (EDX), determination of viscosity and molecular weight by dissolving of cellulose in copper(II)-ethylenediamine complex (CED) solution, and BNC yield, using wet and dry samples weighed.
Results:The results showed that yield of BNC static medium is more than BNC dynamic culture medium. Comparison of FE-SEM images showed that the culture method has a significant effect on the morphology structure of the BNCs fibers produced. Comparison of FE-SEM images of two cultivars showed that the morphology of BNC in static is porous and in dynamic is clustered together. The size of nanoparticles is generally below 60 n.m. for both BNC. The results of XRD test showed that the crystallinity of BNCs in the static and dynamic medium was 72.49% and 14.29%, respectively. Also, viscosity of BNCs in the static and dynamic medium was 110.7 centipoise (cP) and 97 cP, respectively.
Conclusion: In general, with respect to characteristics of BNC products and comparison with other studies, we concluded that suitable BNC is produced in this study that with respect to research needed in this field in the country and medical applications and engineering are important.
Background and objective: Bacterial Nano-cellulose (BNC) due to special microscopic structures has a high potential in special used (such as medical and sanitary applications). BNC differs from plant cellulose in its higher purity, crystallinity, and degree of polymerization. The goal of this study was to investigate the production of BNC using Acetobacter xylinum bacteria and its characteristics evaluation.
Materials and methods: In order to obtain BNC, the bacterium was cultured in a liquid culture medium under static and dynamic conditions and the resulting cellulose was washed and purified. Microstructures with field emission scanning electron microscopy (FE-SEM), crystallinity structure by X-ray diffraction (XRD), weight percentage, size and distribution of BNC with energy-dispersive X-ray spectroscopy (EDX), determination of viscosity and molecular weight by dissolving of cellulose in copper(II)-ethylenediamine complex (CED) solution, and BNC yield, using wet and dry samples weighed.
Results:The results showed that yield of BNC static medium is more than BNC dynamic culture medium. Comparison of FE-SEM images showed that the culture method has a significant effect on the morphology structure of the BNCs fibers produced. Comparison of FE-SEM images of two cultivars showed that the morphology of BNC in static is porous and in dynamic is clustered together. The size of nanoparticles is generally below 60 n.m. for both BNC. The results of XRD test showed that the crystallinity of BNCs in the static and dynamic medium was 72.49% and 14.29%, respectively. Also, viscosity of BNCs in the static and dynamic medium was 110.7 centipoise (cP) and 97 cP, respectively.
Conclusion: In general, with respect to characteristics of BNC products and comparison with other studies, we concluded that suitable BNC is produced in this study that with respect to research needed in this field in the country and medical applications and engineering are important.

Keywords

References

1.Castro, C., Zuluaga, R., Álvarez,C., Putaux, J.L., Caro, G., Rojas,O.J., Mondragon, I., and Ga˜nán, P.2012. Bacterial cellulose produced bya new acid-resistant strain of Gluconacetobacter genus. Carbohydrate Polymers. 8: 4. 1033-1037.

2.Cheng, Z., Yang, R., and Liu, X. 2016. Production of bacterial cellulose by Acetobacter xylinum through utilizing acetic acid hydrolysate of bagasse as
low-cost carbon source. Bioresources.
3.Cho, E.J., Oh, J.Y., Chang, H.Y.,and Yun, J.W. 2006. Production of exopolysaccharides by submerged mycelial culture of a mushroom Tremella fuciformis. J. of Biotechnology. 127: 129-140.

4.Dufresne, A. 2013. Nanocellulose: anew ageless bionanomaterial. Materials Today. 16: 6. 220-227.
5.Esa, F., Masrinda Tasirin, S., and Abd Rahman, N. 2014. Overview of bacterial cellulose production and application. J.
of Agriculture and Agricultural Science Procedia. 2: 113-119.
6.Esa, F., Rahman, N.A., Kalil, M.S., and Tasirin, S.M. 2017. Effects of agitation conditions on bacterial cellulose production by Acetobacter xylinum 0416 in fermentation of matured coconutwater medium. Malaysian J. of Analytical Sciences. 21: 1. 
7.Heinze, T., and Liebert, T. 2012. Celluloses and polyoses/hemicelluloses. Polymer Science: A Comprehensive Reference. 
8.Jahan Latibari, A., Khosravani, A. and Rahmaninia, M. 2007. Paper recycling technology. Tehran: Arvij press, 544p.
(In Persian)
9.Kang, X., Wang, Y., Harvey, L.M.,and Mcneil, B. 2000. Effect of air flow rate on flow scleroglucan synthesis
by sclerotiumglucanicum in an airlift bioreactor with an internal loop. J. of Bioprocess and Biosystems Engineering. 23: 69-74.
10.Klemm, D., Kramer, F., Moritz, S., Lindstrom, T., Ankerfors, M., Gray, D., and Dorris, A. 2011. Nanocelluloses: A new family of nature-based materials. Angewandte Chemie International Edition, 50: 24. 5438-5466.
11.Krystynowicz, A., Czaja, W.,Jezierska, A.W., Mis´kiewicz, M.G., Turkiewicz, M., and Bielecki, S.2002. Factors affecting the yieldand properties of bacterial cellulose.J. of Industrial Microbiology and Biotechnology. 29: 4. 189-195.
12.Krystynowicz, A., Maria, K.,Aginiezka, W.K., Stanislaw, B.,Emilia, K., Aleksander, M., and Andrzej, P. 2005. Molecular basis
of biosynthesis disappearance in submerged culture of Acetobacter xylinum. J. of Industrial Microbiology and Biotechnology. 52: 3. 691-698.
13.Kurtoglu Uzyol, H., and TurkerSacan, M. 2017. Bacterial cellulose production by Komagataeibacter hansenii using algae-based glucose. Environmental Science and Pollution Research. 24: 12. 11154-11162.
14.Mirshokraei, S.A. 2003. Pulp andpaper technology. Aiij Press, 501p. (Translated in Persian)
15.Mirshokraei, S.A. 2002. Wood chemistry: fundamentals and applications. Aiij Press. 198p. (Translated in Persian)

16.Moosavi-Nasab, M., and Yousefi, A. 2012. Investigation of physicochemical properties of the bacterial cellulose produced by Gluconacetobacter xylinus from date syrup. International J.
of Nutrition and Food Engineering.4: 8. 613-618.

17.Morales-Narváez, E., Golmohammadi, H., Naghdi, T., Yousefi, H., Kostiv, U., Horak, D., Pourreza, N., and Merkoci, A. 2015. Nanopaper as an optical sensing platform. ACS Nano. 9: 7. 7296-7305.

18.Pecoraro, E., Manzani, D., Messaddeq, Y., and Ribeiro, S.J.L. 2008. Bacterial cellulose from Glucanacetobacter xylinus: preparation, properties and applications. Monomers, Polymers and Composites from Renewable Resources. Chapter. 17: 3
19.Poletto, M.P., Zattera, A.J., and Santana, R.M.C. 2012. Structural differences between wood species: Evidence from chemical composition, FTIR spectroscopy and thermogravimetric analysis. J. of Applied Polymer Science. 126: 1. 336-343.
20.Rangaswamy, B.E., Vanitha, K.P.,and Hungund, B.S. 2015. Microbial cellulose production from bacteria isolated from rotten fruit. InternationalJ. of Polymer Science. 2015: 1-8.
21.Rezayati Charani, P., and Dehghani-Firouzabadi, M. 2016. Comparison of produced film of cellulose nano-fibers by dried and vacuum filtrated method from unbleached kraft pulp of kenafbast fiber. Iranian J. of Forest and Wood Products. 68: 2. 317-328. (In Persian)
22.Torres, F.G., Commeaux, S., and Troncoso, O.M. 2012. Biocompatibility of bacterial cellulose based biomaterials. Functional Biomaterials. 3: 4. 864-878.

23.Ullah, H., Santos, H.A., and Khan, T. 2016. Applications of bacterial cellulose in food, cosmetics and drug delivery.J. of Cellulose. 23: 4. 2291-2314.

24.Yousefi, H., Faezipour, M., Nishino, T., Ebrahimi, G., and Shakeri, A. 2011. All-cellulosecomposite and nanocomposite made from partially dissolved micro and nanofibers of canola straw. Polymer J.43: 559-564.
Journal of Wood and Forest Science and Technology
Volume 26, Issue 3
June 2020
Pages 29-42

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