Asexual reproduction of hybrid poplar "Mofid" (Populus euphratica Oliv. X P.alba L.) using tissue culture

Abstract
In order to establish in vitro proliferation of new hybrid poplar “Mofid”(Populus euphratica Oliv. × P.alba L.), shoots were collected from mature tree in Research nursery belongs to the department of Research Institute of Forests and Rangelands. Explants with single node were disinfected by different isolation treatment. Mercuric chloride with % 0.1 (W/V) concentrated solutions for 1 minute with 90% survival rate of explants appeared to be suitable disinfection treatments. The establishments of explants were taken in MS medium supplemented with 0.5 mg/l BA and 0.01 mg/l IBA. For evaluation of most affecting factors on in vitro proliferation, the experiment was conducted in a completely randomized design with 9 treatments including three media (MS,WPM and DKW) and three growth regulators hormones (BA+ 0.5 mg/l, 2ip +0.5 mg/l and BA+0.5 mg/l plus 2ip+0.5 mg/l) with three replication. Analysis of variance indicated that there is significant differences between media for maxillary shoot proliferation at α=0.5% level. Highest shoot proliferation ( 3.2 mean number of new induced shoots per explant) and shoot elongation (1.465 cm mean height of shoots) have been observed for DKW medium containing 0.5 mg/l 2iP plus 0.5 mg/l BA. Numerous micropropagation circles have been initiated by in vitro nodal segments of shoots. Roots were induced in DKW hormone free medium after two weeks. Acclimatization of rooted plantlets took place in polyethylene caps jam bottle, containing a light soil (Peat, hummus and sand in equal percentages) in growth chamber. Successfully acclimatized plantlets transferred to field.