Comparison of Peroxidase Seasonal Alteration Among Populus deltoides Clones

Plantation of fast-growing species such as Poplar is one of the important methods for wood production in the countries with low natural forest areas. Physiological differences of Populus deltoides clones planted through three decades in Shast Kalate research forest are unknown. Peroxidase enzyme in this research was used for clone separation as a biochemical marker that is sensitive to environmental alteration especially temperature and its direct participation in lignification process. Sampling was done from one year old seedlings of five clones and quantitative and qualitative activities of peroxidase during five months (September through May) were measured using Worthington and PAGE methods, respectively. The results showed that peroxidase quantitative activity and isoenzyme pattern of clones were different among the months which indicated the role of this enzyme in cold hardiness and lignification of the clones.