Micropropagation of Crataegus pseudoheterophylla Pojark. via in Vitro Culture

Ahmadloo, Fatemeh; Tabari, Masoud; Azadi, Pejman; Hamidi, Aidin; Beiramizadeh, Ebrahim

Micropropagation of Crataegus pseudoheterophylla Pojark. via in Vitro Culture

Document Type : Complete scientific research article

Authors

¹ Faculty of Natural Resources, Tarbiat Modares University

² author and Associate Professor of Forestry, College of Natural Resources, Tarbiat Modares University. Noor, Iran.

³ Assistant Professor,Agricultural Biotechnology Research institute of Iran (Karaj)

⁴ Assistant Professor, Seed & Plant Certification & Registration Institue

⁵ Scientific board members, Department of Biotechnology, National Research Center of Ornamental Plants, Mahallat, Iran

Abstract

Crataegus from Rosaceae family is a tree which has medical, ornamental and commercial utilizations. In this Study, all stages of Micropropagation of Crataegus pseudoheterophylla Pojark. including sterilization, establishment, proliferation and rooting were evaluated. For disinfection, explants were taken in spring and autumn and rinsed under running tap water for 30 min then dipped in 70% ethanol for 30 s followed by immersion in ClONa 1.5% and 2% for 10, 15 and 20 minutes. MS, WPM and DKW media were used for initial establishment. Proliferation was conducted on MS and WPM medium supplemented with different concentrations of BAP and NAA and rooting of shoots was done in 1/2 MS medium supplemented with different concentrations of IBA. Results indicated that the best method of sterilization was obtained in ClONa 1.5% for 15 minutes in spring and the best medium of establishment was obtained on MS medium with maximum shoot length (3.5 cm) and the survival rate (90.33%). The best treatment for proliferation was 8 mg / l BAP plus 2 mg / l NAA with 39.33 shoot numbers on MS medium and the best treatment for rooting was 1 mg / l IBA with 25% rooting on 1/2 MS medium supplemented with 40 mg / l PG.

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