Comparison of the efficiency of leaf morphological and Peroxidase isozyme markers in segregation of Fagus orientalis Lipsky plus and none-plus trees in Shastkalateh forest-Gorgan

Mahmoodi, Mohammadbagher; Azadfar, Davoud

Comparison of the efficiency of leaf morphological and Peroxidase isozyme markers in segregation of Fagus orientalis Lipsky plus and none-plus trees in Shastkalateh forest-Gorgan

Document Type : Complete scientific research article

Authors

Ms.c graduate

Abstract

Exploitation and climate change factors affected on beech trees (Fagus orientalis L.) so, selection and preservation of plus trees is very important for the protecting and improving of the beech stands.In order to this goal, this study was done separation of plus and none-plus trees in the same ecological conditions by peroxidase isozyme and leaf morphological markers. In July 2011, the leaf and branch samples were collected from outer layers of crown. All the materials were sampled from south aspect of plus and none plus tree (at the same height- about 10 meters) groups of beech stands in Shast Kalate forest of Gorgan. The qualitative activity of peroxidase was assessed by PAGE method. Also for investigation of leaf morphology, 10 leaf morphological traits were measured. Peroxidase banding pattern showed 8 and 11 bands in branch and leaf organs respectively, in three zones of polyacrilamide gel. The both markers showed an acceptable efficiency for separation of plus and none plus trees. Although results of the used markers were in line together, ability of leave and branch Peroxidase isozyme were better than leaf morphology marker.

Keywords